View and download qiagen tissuelyser ii user manual online. Qiagen tissuelyser 2 retch mm400 sample preparation. May 2016 tissuelyser ii user manual gtac home page. Tissueruptor tissuelyser lt tissuelyser ii throughput samplesrun 1 up to 12 up to 48 or 192 disruption technology rotorstator bead mill bead mill disruption time 30 seconds 40 seconds to 5 minutes 20 seconds to 5 minutes table 1. These quaternary test strips comply with federal, state, and local health regulations. The tissuelyser ii disrupts multiple biological samples through highspeed shaking and is wellsuited for disruption of human, animal and plant tissues, as well as bacteria and yeast. Working in a chemical fume hood, transfer the homogenized sample by pipet to a new 2 ml screw cap tube containing 0.
Alternatively, a grinding jar set can be used to process large samples. For highthroughput disruption of biological samples. Iris receives 510k for body fluids analysis using iq 200. These quaternary test strips read 0, 150, 200, 400, and 500 ppm. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. All instruments are reconditioned and include a 90 day warranty on parts and labor. Mix well by inverting the tube several times do not vortex. Tissuelyser lt handbook qiagen gel electrophoresis. We stiil use pestle and mortar which is very timeconsuming and we would like to speed. Standard assay protocol i luminometers without injectors. The volume of the tube must be at least twice that of the buffer rlt used. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation.
Standard assay protocol i luminometers without injectors e3300 protocol. High level of agreement between cp3000 and five other. Equipment for sale category or equipment item tissuelyser beadhomogenizer not found. Qiaex ii handbook qiaex ii agarose gel extraction protocol. Mg scientific 8500 107th street pleasant prairie wi 53158 8003438338. Pathway assay kit active bcatenin dephospho dp s33s37t41 instructions manual for research use only introduction. Dna yields were determined using a spectrophotometer. Novus bcatenin pathway assay kit has been designed to detect the active dephosphorylated form of bcatenin.
The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation, such as the qiasymphony sp. Safety information 6 tissuelyser ii user manual 042010 with accessories approved by qiagen see section 3. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Always read the product label before use and check with your health professional before using this product. These units measure concentration of quaternary sanitizers. Draft opinion on use of the threshold of toxicological. It ramps up to 30 shakes per second and the timer works as it should. Apr 22, 2016 when 2 patients with the attenuated type and 2 patients with the severe type of mps ii, all of them were antibodypositive, were compared, the concentrations of hs in the severe type of mps ii 151. These quaternary test strips provide reliable results. I tried a tissue lyser from qiagen to disrupt some mice aortas in order to extract protein. The tissuelyser ii can be used for medium to highthroughput sample disruption for molecular analysis. For optimal operation, the tissuelyser must always be balanced. Paraffin blocks will be returned with final report. Timerun, 1 samplerun, qiagen kits for realtime pcr, the rotorgene q can be used for a wide range of applications.
Supplied as a readytouse 2x master mix, qmax green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Do not continue to use the tissuelyser ii or tissuelyser accessory if it is damaged. Qiaquick pcr purification kit 250, 28106 from qiagen labsave. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Qiagen tissuelyser ii retsch mm400 mixer mill grinder. Stefan fischerfruhholz, february 15, 2012 senior product manager membrane chromatography. Excise the tissue sample from the animal or remove it from storage. Sample disruption can be carried out at room temperature or after storing the adapter on dry ice for at. Tissuelyser lt adapter this adapter allows disruption of 12 samples in parallel using standard 2 ml microcentrifuge tubes, such as sample tubes rb 2 ml cat. I am sure you can buy equivalent beads at vwr or fisher. Patient collection procedure guide carefully insert the swab into the opening of the vagina, about 2 inches 5 cm, and gently rotate the swab for 10 to 30 seconds. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. It has been subjected to severe transport stresses. For mediumtohighthroughput sample disruption for molecular analysis.
This small bead mill has high throughput with highspeed shaking for the disruption or homogenization of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. Tissuelyser ii user manual 052016 7 it or the line power cord appears to be damaged. Rna isolation with trizol invitrogen and qiagen rnaeasy. The qiagen tissuelyser lt sample disruptor provides quick and efficient disruption in up to 12 samples. Oct 23, 20 the cell pellets were then snap frozen in liquid nitrogen, homogenized by cryomilling after addition of a 5mm steel bead using a tissue lyser qiagen tissuelyser ii at 25 hz for 15 s 46 times, and the powder was either processed directly or stored in liquid nitrogen. Aug 29, 2017 qiagen qiagility pcr system bostonind 14253 duration. Do not continue to use the tissuelyser ii or tissuelyser. Determine the amount of tissue and place it into the qiazol lysis reagent. Does anyone have experience with tissue lyser from qiagen. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment, 40mers fragments removed, ideal for sequencing, microarray analysis, ligation and transformation, restriction. Advanced nutritional innovations hydrion saliva ph test. Various rat tissues were disrupted using the tissuelyser lt or. Disrupt cells in the tissuelyser for 5 min at maximum speed.
Lung 60 50 40 30 20 10 0 tissuelyser lt tissuelyser ii liver heart brain skin 400 300 200 100 0 heart brainlung tissuelyser lt tissuelyser ii figure 6. Equipnet is constantly receiving used qiagen equipment through. Tissuelyser lt adapter, 12tube from qiagen selectscience. Qiagen provides 2 systems for fast, effective sample disruption. Qiagen focuses on breaching great success and development in their company to better serve their consumers. To measure the concentration of quaternary sanitizers, specifically alkyldimethylbenzyl ammonium compounds, rocall ii and hyamine 3500 includes one 15 ft. Instrument information would you like to receive more information about a specific qiagen instrument. Advanced nutritional innovations saliva ph test papers 1 rolls every day, saliva ph test papers is intended for testing body ph by testing saliva ph. It has been stored under unfavorable conditions for a prolonged period. Servicing of the tissuelyser ii should only be performed by qiagen or an authorized agent. Make sure the swab touches the vaginal walls so that moisture is absorbed by the swab. These are available online in convenient and compact pdf.
B rna was purified from 20 mg samples on the qiacube using the rneasy fibrous tissue mini kit skin, heart, and lung or rneasy lipid tissue mini kit brain. A supplementary protocol for simultaneous disruption of up to 48 tissue samples using the tissuelyser can be obtained by contacting qiagen technical services see back cover. Rnalater rna stabilization reagent, tissuelyser ii. Used qiagen tissuelyser ii 2030242 dna purification. Lt is comparable to that achieved with the wellestablished tissuelyser ii figures 6 and 810. All instructions and protocols in this handbook apply to both the tissuelyser ii and the. Qiagen tissuelyser ii tissue disruption systemretsch mm400 mixer mill grinderincludes 2 x 24 well qiagen tissuelyser tube adapter sets 2 x 24 2 sets of adapter plates and 2 racks for use with 1. Laboratory equipment for sale scientific equipment repair.
Qiagen is the leading provider of innovative sample and assay technologies, enabling. Qiagen tissuelyser ii sample disruption preparation bead. I asked precellys similar question and they suggested 2 3 cycles 40 second each with ck28 beads ceramic 2. The tissuelyser adapter set 2 x 24 contains a pair of tl reactiontube holders, 24tube. The levels of urinary glycosaminoglycans of patients with. The tissuelyser ii is an integral part of qiagen s complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. The tissuelyser system provides convenient, simultaneous disruption and homogenization of multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads.
We recommend that you contact an equipnet sales representative to set up your own inspection. For purification of up to 10 micro g pcr products, 100 bp to 10 kb. Qiagen tissuelyser ii sample disruption 11843 youtube. Her2, breast, quantitative immunohistochemistry, automated. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation in cell culture. The tissuelyser ii is designed for medium to high throughput disruption of human, animal and plant tissues as well as bacteria and yeast samples.
Does anyone use the tissuelyser ii qiagen equipment to. Study trident technical college biology 210 flashcards and notes. Qiagen offers advanced and innovative solutions for all of your assay and sample equipment needs. Cookies this website uses cookies, which are necessary to is functionling and required to achieve the purposes illustrated in the cookie policy. Compact homogenizer for medium to larger sample series adapters 2 x 24 2ml or 2 x 96 1. Place the tube on wet ice temporarily until ready to proceed to. For disruption of up to 48 or 192 biological samples. This german made shaker appears in good condition without cracks in the plastic cover or other damage to the housing or workings. Other methods of mechanical disruption can be used instead.
I used it at maximal speed, as these tissue are really elastic and difficult to disrupt. Tissuelyser ii laboratory equipment pdf manual download. Iris international, a manufacturer and marketer of automated invitro diagnostic urinalysis systems and medical devices used in hospitals and clinical reference laboratories, has received 510k clearance from the fda to market its new body fluids module for analyzing cerebrospinal and serous body fluids utilizing the companys iq200 product platform. Her2 immunohistochemistry testing on intracystic papillary carcinoma and solid papillary carcinoma, without clearly stating invasive carcinoma, is not appropriate and will be canceled without processing.
Cp3000 and five other coagulation analyzers for inr in a multicenter study july 2017 abbott diagnostics, abbott park, illinois 60014. Biology 210 at trident technical college online flashcards. Usually after dna purification, 260280 ratio will ranging between 1,8 2 pure dna but all of my purification result shows 260280 ratio higher than 2 between 2 2,5. Sample tubes rb 2 ml x 2 ml sample tubes, for tissuelyser lt qiagen 990381 reagent dx qiagen 19088 allprep kit mini qiagen 80204 allprep kit micro qiagen 80284 fisherbrand textured nitrile gloves large fisher 27005853 ice bucket green fisher 1167636 wet ice in house na na nuclease free 2. If the tissuelyser ii is not in use, switch it off at the power switch.
Tissuelyser adapter sets are designed for efficient and userfriendly disruption of up to 48 or 192 samples in parallel using the qiagen tissuelyser ii. If stored at 28c products a1153, a4529 and a3428 have a shelflife of two years. This easytouse instrument automates purification of dna, rna, and protein from 196 samples. Tissueruptor and tissuelyser ii disruption systems biomarker. Equipnet is constantly receiving used qiagen equipment through our exclusive contracts with our clients. The threshold of toxicological concern ttc approach is a risk assessment tool that is based on the principle of establishing a human exposure threshold value for chemicals, below which there is a very low probability of adverse effects to human health. The tissuelyser lt is compact so you can keep it nearby for easy access. There are minor cosmetic scratches on the top plate, but nothing serious. Each holder holds up to 24 microcentrifuge tubes 2 ml. If using the tissuelyser lt or tissuelyser ii, add the stainless steel beads of choice 1 x 5 mm or 2 x 7mm to the tube before the addition of qiazol. Micro essential lab hydrion quaternary test paper qt40 with. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide. The process is based on bead mill technology, with highspeed shaking in plastic tubes with stainless steel, tungsten, carbide or glass beads, which are 3 mm, 5 mm or 7 mm in diameter. Various rat tissues were disrupted using the tissuelyser lt or tissuelyser ii.
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